The ZymoPURE II™ range from Zymo Research enables the isolation of ultra-pure, vaccine/transfection grade plasmid DNA from E. coli using the fastest and easiest method on the market. Featuring ZymoPURE II technology along with a modified alkaline lysis system and novel binding chemistry, these kits can isolate up to 400 µg of vaccine/transfection grade, salt, protein and RNA-free plasmid DNA in less than 20 minutes.
Benefits of using the ZymoPURE II plasmid isolation kits
• Vaccine/transfection grade ultra-pure DNA produced
• 20-minute protocol (Midiprep and Maxiprep)
• Up to 3 µg/µl plasmid DNA isolated
• Endotoxin levels of less than 0.025 EU/µg plasmid DNA (if using additional EndoZeroTM columns provided)
• Simple protocol
• Reliable and consistent performance
• DNA ready for immediate use
• Wide downstream application compatibility
• Sample kits available (Midiprep and Maxiprep)
ZymoPURE Plasmid Kit formats available:
| Catalogue number | Product | Highest yield/binding capacity | Elution volume | Culture input | Endotoxin levels | Format |
|---|---|---|---|---|---|---|
| D4212 D4211 D4210 D4208T D4209 |
ZymoPURE Plasmid Miniprep | 100 µg | ≥ 25 µl | ≤ 5 ml culture | ≤ 1 EU/µg DNA | Spin column |
| D4200 D4201 |
ZymoPURE II Plasmid Midiprep | 400 µg | ≥ 100 µl | ≤ 50 ml | ≤ 0.025 EU/µg DNA | Spin column |
| D4203 D4202 |
ZymoPURE II Plasmid Maxiprep | 1.2 mg | ≥ 200 µl | ≤ 150 ml | ≤ 0.025 EU/µg DNA | Spin column |
| D4204 | ZymoPURE II Plasmid Gigaprep | 10 mg | ≥ 2 ml | ≤ 2.5 l | ≤ 0.025 EU/µg DNA | Spin column |
| D4213 | ZymoPURE-Express Plasmid Midiprep | 1.2 mg | ≥ 200 µl | 25 ml | ≤ 1 EU/µg Plasmid DNA | Spin column |
