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ZymoPURE II Plasmid Midiprep and Maxiprep kits

The ZymoPURE II™ Plasmid midiprep and plasmid maxiprep kits from Zymo Research enable the isolation of up to 400 µg (midiprep) or 1.2 mg (maxiprep) of salt, protein and RNA-free vaccine/transfection quality plasmid DNA from E. coli in less than 20 minutes.

For a limited time only, in celebration of Zymo's 30th anniversary, we are offering 20% off midi and maxiprep ZymoPURE kits.

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Benefits of using the ZymoPURE II Plasmid Maxiprep & Midiprep kits
• Ultra-pure vaccine/transfection grade DNA produced
• 20 minute protocol
• Up to 400 µg (midiprep)/1.2 mg (maxiprep) plasmid DNA isolated
• Endotoxin levels of ≤ 0.025 EU/µg Plasmid DNA (if using additional EndoZero™ columns provided)
• Simple protocol
• Reliable, consistent performance
• DNA ready for immediate use
• Wide downstream application compatibility
• Free samples available to trial

Simple 20 minute EndoZero Midi/Maxi preps

ZymoPURE II Midiprep & Maxiprep 20 minute protocol

ZymoPURE plasmid midiprep & maxiprep kits produce ultra-pure, vaccine-grade plasmid DNA 

Ultra-Pure Plasmid DNA In 20 Minutes – ZymoPURE II™

Stated manufacturers’ endotoxin levels for the ZymoPURE II Maxiprep kit compared to two separate kits from Supplier Q.
*Endotoxins <0.04 EU/μg of plasmid DNA meets the FDA limit for vaccines

ZymoPURE II Plasmid Maxiprep kit

  ZymoPURE II™ Maxiprep kit Supplier Q Endofree Maxiprep Supplier Q Maxiprep
Maximum yield 1200 µg 500 µg 500 µg
Endotoxins ≤ 0.025 EU/µg ≤ 0.1 EU/µg ≤ 10 EU/µg
Time per prep < 20 min 150 min 160 min
Hands-on time 15 min 45 min 45 min
Loads via vacuum or centrifuge (no gravity flow)  Yes No No
Elute from a spin column (no precipitation) Yes No No
Vaccine grade* Yes No No
In vivo & gene therapy grade Yes Yes No
Transfection grade (sensitive & primary cells) Yes Yes No

* Endotoxins below FDA limit for vaccines <0.04 EU/µg of plasmid DNA

ZymoPURE plasmid midiprep testimonials
“With your kit, I got twice the amount of DNA that I usually get with Invitrogen's midi kit, in half the time. Can't beat that!”
D. G., Immunology Consultants Laboratory

“I have used the Promega and Qiagen kits, but even with following all the steps carefully, I have never got such high quality and concentrated DNA.”
P. J., University of Miami

ZymoPURE plasmid maxiprep Testimonials
“We were impressed by the high yield of plasmid DNA from this kit! From a 150 ml culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”
C. S., Abbott Laboratories

“DNA obtained from using the ZymoPURE kit was ten times the amount of DNA obtained from using the QIAGEN kit.”
J. M., Vanderbilt University

Free samples
Request a free ZymoPURE II Midiprep sample
Request a free ZymoPURE II Maxiprep sample

Material available to download
ZymoPURE II Plasmid Midiprep protocol
ZymoPURE II Plasmid Maxiprep protocol
ZymoPURE II plasmid purification kits datasheet

Lipofectamine is a registered trademark of Life Technologies Corporation. ONE-Glo is a trademark of Promega Corporation.

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Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
ZymoPURE II™ Plasmid Midiprep D4200 · Zymo Research popular product
Zymo Research
25 preps £372.00
25 preps
ZymoPURE II™ Plasmid Midiprep D4201 · Zymo Research popular product
Zymo Research
50 preps £669.00
50 preps
ZymoPURE II™ Plasmid Maxiprep D4202 · Zymo Research popular product
Zymo Research
10 preps £305.00
10 preps
ZymoPURE II™ Plasmid Maxiprep D4203 · Zymo Research popular product
Zymo Research
20 preps £576.00
20 preps