The ZymoPURE II™ Plasmid midiprep and plasmid maxiprep kits from Zymo Research enable the isolation of up to 400 µg (midiprep) or 1.2 mg (maxiprep) of salt, protein and RNA-free vaccine/transfection quality plasmid DNA from E. coli in less than 20 minutes.
Benefits of using the ZymoPURE II Plasmid Maxiprep & Midiprep kits
• Ultra-pure vaccine/transfection grade DNA produced
• 20 minute protocol
• Up to 400 µg (midiprep)/1.2 mg (maxiprep) plasmid DNA isolated
• Endotoxin levels of ≤ 0.025 EU/µg Plasmid DNA (if using additional EndoZero™ columns provided)
• Simple protocol
• Reliable, consistent performance
• DNA ready for immediate use
• Wide downstream application compatibility
• Free samples available to trial
Simple 20 minute EndoZero Midi/Maxi preps
ZymoPURE plasmid midiprep & maxiprep kits produce ultra-pure, vaccine-grade plasmid DNA
Stated manufacturers’ endotoxin levels for the ZymoPURE II Maxiprep kit compared to two separate kits from Supplier Q.
*Endotoxins <0.04 EU/μg of plasmid DNA meets the FDA limit for vaccines
ZymoPURE II Plasmid Maxiprep kit
ZymoPURE II™ Maxiprep kit | Supplier Q Endofree Maxiprep | Supplier Q Maxiprep | |
---|---|---|---|
Maximum yield | 1200 µg | 500 µg | 500 µg |
Endotoxins | ≤ 0.025 EU/µg | ≤ 0.1 EU/µg | ≤ 10 EU/µg |
Time per prep | < 20 min | 150 min | 160 min |
Hands-on time | 15 min | 45 min | 45 min |
Loads via vacuum or centrifuge (no gravity flow) | Yes | No | No |
Elute from a spin column (no precipitation) | Yes | No | No |
Vaccine grade* | Yes | No | No |
In vivo & gene therapy grade | Yes | Yes | No |
Transfection grade (sensitive & primary cells) | Yes | Yes | No |
* Endotoxins below FDA limit for vaccines <0.04 EU/µg of plasmid DNA
ZymoPURE plasmid midiprep testimonials
“With your kit, I got twice the amount of DNA that I usually get with Invitrogen's midi kit, in half the time. Can't beat that!”
D. G., Immunology Consultants Laboratory
“I have used the Promega and Qiagen kits, but even with following all the steps carefully, I have never got such high quality and concentrated DNA.”
P. J., University of Miami
ZymoPURE plasmid maxiprep Testimonials
“We were impressed by the high yield of plasmid DNA from this kit! From a 150 ml culture, I was able to isolate 0.912 mg DNA, in the same amount of time (and effort) I could get about half as much DNA from the comparable Qiagen kit.”
C. S., Abbott Laboratories
“DNA obtained from using the ZymoPURE kit was ten times the amount of DNA obtained from using the QIAGEN kit.”
J. M., Vanderbilt University
Free samples
Request a free ZymoPURE II Midiprep sample
Request a free ZymoPURE II Maxiprep sample
Material available to download
ZymoPURE II Plasmid Midiprep protocol
ZymoPURE II Plasmid Maxiprep protocol
ZymoPURE II plasmid purification kits datasheet
Lipofectamine is a registered trademark of Life Technologies Corporation. ONE-Glo is a trademark of Promega Corporation.
Products
Note: product availability depends on country - see product detail page.
Details | Cat number & supplier | Size | Price |
ZymoPURE II™ Plasmid Midiprep D4200 · Zymo Research | D4200 Zymo Research |
25 preparations |
£297.00
25 preparations
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ZymoPURE II™ Plasmid Midiprep D4201 · Zymo Research | D4201 Zymo Research |
50 preparations |
£529.00
50 preparations
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ZymoPURE II™ Plasmid Maxiprep D4202 · Zymo Research | D4202 Zymo Research |
10 preparations |
£243.00
10 preparations
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ZymoPURE II™ Plasmid Maxiprep D4203 · Zymo Research | D4203 Zymo Research |
20 preparations |
£468.00
20 preparations
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