The ZymoPURE II™ plasmid gigaprep kit enables the isolation of up to 10 mg of salt-, protein- and RNA-free vaccine/transfection quality plasmid DNA from E. coli in less than 50 minutes. As an added convenience, this gigaprep kit contains coloured buffers enabling error-free visualisation and identification of complete bacterial cell lysis and neutralisation.
Benefits of using the ZymoPURE II plasmid gigaprep kit
• Ultra-pure DNA produced
• Simple, 50 minute protocol
• Up to 10 mg plasmid DNA isolated
• Endotoxin levels of ≤ 0.025 EU/µg Plasmid DNA (if using additional EndoZero™columns provided)
• Reliable, consistent performance
• DNA ready for immediate use
• Wide downstream application compatibility
ZymoPURE II plasmid gigaprep kits use the simplest workflow
ZymoPURE II plasmid kits provides the highest recovery of plasmid DNA
Plasmid DNA concentration and yield from the ZymoPURE II maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E.coli culture grown overnight following the manufacturer's suggested protocol (in duplicate). 1 µl of eluted plasmid DNA was visualised post agarose gel electrophoresis. M: ZR 1 kb DNA Marker (Zymo Resarch).
ZymoPURE II plasmid kits offer the lowest endotoxin levels
Stated manufacturers’ endotoxin levels for the ZymoPURE II Maxiprep kit compared to two separate kits from Supplier Q. Endotoxins <0.04 EU/μg of plasmid DNA meets the FDA limit for vaccines.
Samples are available for the ZymoPURE II Midiprep or Maxiprep. Request a free sample.
Material available to download
ZymoPURE II Plasmid Gigaprep protocol
ZymoPURE II plasmid purification kits datasheet
Note: product availability depends on country - see product detail page.