ZymoBIOMICS HMW DNA Standard

The ZymoBIOMICS™ HMW DNA Standard provides a solution for routine quality control and benchmarking the performance of long read/3rd generation sequencing for microbiomics and metagenomics analysis. Consisting of a mixture of high molecular weight genomic DNA from seven bacterial and one fungal strain, this standard has an accurately defined composition, negligible impurities (<0.01%) and contains genomes of a wide range of GC content (15%-85%).

Unlike other microbial standards which contain fragmented DNA due to mechanical homogenisation-based DNA extraction, the ZymoBIOMICS HMW standard offers intact, >50 kb DNA.

Benefits of using ZymoBIOMICS HMW DNA Standard
• Assess performance & serve as a routine quality control for 3rd generation sequencing (e.g. Oxford Nanopore® & PacBio®)
• >50 kb size
• Long-read sequencing ready
• 100 ng/µl concentration
• Certified <0.01% foreign microbial DNA
• Certified <15% average relative-abundance deviation

High-quality long read sequencing

Long read sequencing was performed on Oxford Nanopore MinION™ using the Ligation Sequencing kit (LSK 109) for library preparation. Basecalling quality of number of reads shows overall good q-score of 11.

Material available for download
ZymoBIOMICS HMW DNA Standard protocol

Also available is the Quick-DNA™ HMW MagBead kit for purification of DNA up to 150 kb optimised for long read sequencing.

PacBio® is a trademark of Pacific Biosciences®.
Oxford Nanopore® and MinION™ are trademarks of Oxford Nanopore Technologies.