High molecular weight DNA magbead kit: Quick-DNA

The Quick-DNA™ high molecular weight (HMW) Magbead kit from Zymo Research simplifies HMW DNA extractions from any sample. DNA produced is high quality, up to 150 kb and optimised for long read sequencing (including Oxford Nanopore™ and PacBio SMRT™ sequencing). High purity DNA can be obtained even from challenging high biomass samples such as blood or faeces. 

Benefits of using the Quick-DNA HMW kit
•    Get up to 150 kb ultra-pure DNA from any sample
•    Achieve high purities even in difficult high biomass samples e.g. blood or faeces
•    Streamlined HMW DNA purification for long-read sequencing
•    HMW bacterial DNA without bead-beating
•    Validated for all third-generation sequencing platforms including Oxford Nanopore™ and PacBio SMRT™ sequencing

Longer DNA for longer read lengths

High Molecular Weight DNA. Cultured E. coli (~10⁸ cells), Cultured L. monocytogenes (~10¹⁰ cells), 50 mg feces, and 75 µl ZymoBIOMICS® Microbial Community Standard (D6300) were input into the Quick-DNA™ HMW MagBead kit (n=2). Length of the highest detectedpeak were recorded and averaged for each sample. DNA size was analysed using Aligent’s Femto Pulse system.

Ultra-pure DNA

Ultra-Pure. 200 µl human blood, 50 mg feces, 25 mg mouse gut, and 75 µl ZymoBIOMICS Microbial Community Standard (D6300) were input into the Quick-DNA HMW MagBead and Supplier Q kits (n=2). Zymo Research consistently had higher purities (A260/230: >1.8) and comparable DNA recovery (µg).Absorbance A260/230, and total DNA recovery (µg) were quantified by NanoDrop™ 2000. 

ZymoBIOMICS HMW DNA Standard
The ZymoBIOMICS High Molecular Weight (HMW) DNA Standard consists of 7 bacterial strains (3 gram-negative and 4 gram-positive) and 1 yeast, providing a solution for assessing performance of information-rich 3rd generation sequencing and metagenomic measurements (e.g. Oxford Nanopore™ & PacBio™). All current microbial standards contain fragmented DNA, typically due to mechanical homogenisation-based DNA extraction. However, the ZymoBIOMICS HMW standard offers intact, >50 kb DNA to enable nucleotide reading at the single molecule level as required by 3rd generation sequencing. Find out more. 

High quality DNA is ready for all sensitive downstream applications such as:
•    Long read sequencing
•    PCR
•    Endonuclease digestion
•    Southern blotting
•    Genotyping
•    Next-Generation Sequencing
•    Bisulfite conversion, etc. (A260/A230 ≥ 1.8)

Material available for download
Quick-DNA HMW MagBead kit protocol

Oxford Nanopore is a trademark of Oxford Nanopore Technologies
PacBio SMRT sequencing and PacBio are trademarks of Pacific Biosciences