TransIT®-BrCa from Mirus Bio is a high performance, low toxicity DNA transfection reagent for breast cancer cell types. As the first reagent available that is wholly dedicated to cell line specific breast cancer lines, TransIT-BrCa efficiently delivers DNA to many breast cancer cell types whilst maintaining low cellular toxicity levels and reduced experimental bias.
Benefits of using TransIT-BrCa
• Breast cancer cell optimised
• High expression levels in MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D
• High efficiency
• Low cellular toxicity
• Cell density maintained
• Experimental bias reduced
• Superior transfection efficiency
• TransIT-BrCa outperforms Lipofectamine® 2000 in multiple breast cancer cell lines
• Free samples available
"I set up a transfection of ZR-75-1 breast cancer cells (just GFP) to compare the results between our peptide, Lipofectamine 2000 and the Mirus Bio TransIT BrCa reagent. As is evident in my image, the TransIT BrCa reagent performed really well and I will be planning a large scale study in the near future."
Cian McCrudden, Research Fellow, School of Pharmacy, Queen's University, Belfast
TransIT-BrCa exhibits higher luciferase expression in breast cancer cells compared to other transfection reagents
Breast cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression measured at 24 hours post-transfection using a standard assay. Error bars represent standard deviation of triplicate wells.
TransIT-BrCa delivers plasmid DNA with high efficiency
TransIT-BrCa used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections performed in 35 mm Mattek dishes using 4 µl of TransIT-BrCa to deliver 2 µg of DNA (2:1 reagent:DNA ratio). Images captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.
TransIT-BrCa yields high efficiency plasmid DNA transfection
Request a free TransIT-BrCa sample
Material available for download
TransIT-BrCa quick reference protocol
TransIT-BrCa optimisation protocol on 12-well plate
Lipofectamine is a registered trademark of Life Technologies , Inc.
FuGENE is a registered trademark of Fugent, LLC, USA
Guava is a registered trademark of MERCK KGAA
Note: product availability depends on country - see product detail page.
|Details||Cat number & supplier||Size||Price|
|TransIT®-BrCa Transfection Reagent MIR 5504 · Mirus||
|TransIT®-BrCa Transfection Reagent MIR 5500 · Mirus||
|TransIT®-BrCa Transfection Reagent MIR 5505 · Mirus||
|5 x 1 ml||
5 x 1 mlview
|TransIT®-BrCa Transfection Reagent MIR 5506 · Mirus||
|10 x 1 ml||
10 x 1 mlview