The dynamic duo benefits
Total RNA comprises large amounts of undesired ribosomal RNA (rRNA), limiting access to RNAs of interest. RiboCop enables efficient, non-enzymatic depletion of rRNA whilst CORALL provides your rapid, fragmentation-free total RNA sequencing, making this dynamic duo ideal for handling degraded and FFPE RNA.
The bundle offer
1. CORALL V2: Your all-in-one total-RNA sequencing kit
Fragmentation-free, total RNA-seq workflow in <4.5 hours. Offering a standardised workflow across a wide input range (1ng - 1000ng), as well as built-in UMIs and 384 UDIs for maximized sequencing output.
2. RiboCop: Ribosomal RNA depletion kit
Specific, highly effective, non-enzymatic depletion of rRNA from a broad range of sample types. It also helps to retain valuable RNAs not/partially accessible through mRNA-Seq such as; lncRNA / lincRNA, snRNA / snoRNA, circRNA, eRNA.
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CORALL: All-in-one total RNA-seq kit |
RiboCop: rRNA depletion kit |
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Applications
FFPE / Biobank samples: Cancer research
RiboCop rRNA depletion does not rely on enzymatic digestion (no RNase H), so avoids RNA fragmentation. CORALL RNA-Seq produces a fragmentation-free library prep and it's displacement/stop technology is suitable for any sample quality. This improves the analysis of challenging samples for cancer research and diagnosis.
- Degradation-free rRNA depletion and library prep preserve samples from further fragmentation!
- Improved accuracy with built-in UMIs for excellent results from low-quality samples.
- Up to 384 UDIs included for high-level multiplexing and improved demultiplexing.
- No changes to the standard workflow are required
- Ready-to-sequence
libraries in < 6 hours.
CORALL x RiboCop in action
Figure A) highlights how the use of RiboCop alongside CORALL successfully retains high gene discovery rates in human liver RNA-derived FFPE samples when compared to fresh frozen (FF) samples. The number of detected genes is plotted against the total number of reads mapping uniquely to exons. B) Overlap of detected genes at normalized expression levels >5 CPM (for uniquely mapping reads).
Biomarker analysis and circRNAs: cancer research
RiboCop interacts with miRNAs, proteins, translation and modification which are all important Biomarkers in cancer and other diseases.
- circRNAs can be analyzed from blood samples
- Lexogen provide a custom data analysis pipeline available for circRNA analysis using CORALL library preps
- Services options also available!
Whole blood RNA-Seq: cancer research, personalised medicine and biomedical research.
Whole blood RNA is comprised of ~80–90% rRNA as well as undesired globin mRNA, accounting for 30–80% of all mRNAs in the sample.
- Compatible with blood samples stored in PAXgene tubes
- Poly(A) enrichment (optional!)
- Combined rRNA and globin removal
- Efficient globin removal and increased sensitivity for whole blood samples
. - Improved accuracy with built-in UMIs for all input qualities and quantities.
- Up to 384 UDIs for superior error correction capacity for highest quality data
CORALL x RiboCop in action
The Figure shows the increased gene detection for CORALL total RNA-Seq with rRNA depletion and rRNA and globin depletion when compared to untreated whole blood RNA. The number of detected genes is shown as a function of the number of reads uniquely mapping to exons.
RNA therapeutics: cell culture, organoids
Make the most of enzyme-free rRNA depletion and fragmentation-free CORALL RNA-Seq with superior end-to-end coverage and start site representation for base-level isoform resolution.
- Don’t compromise on sensitivity: excellent sensitivity even for samples <10 ng input.
- Improved accuracy with built-in UMIs, especially for low-input samples.
- 384 UDIs for superior error correction capacity for the highest quality data
- Upscale experiments with ease with CORALL's fast and automation-friendly protocol.
CORALL x RiboCop in action
The Figure highlights the kits' exceptional performance on low-input RNA. A) Excellent correlation for replicates of 1ng Universal Human Reference RNA (UHRR) processed with CORALL mRNA-Seq V2. B) Read distribution for 1ng input RNA for CORALL mRNA-Seq V2 and CORALL Total RNA-Seq V2 with RiboCop rRNA depletion
Products
Note: product availability depends on country - see product detail page.
