The CORALL™ Total RNA-Seq V2 Library Prep kits from Lexogen enable easy and cost-efficient generation of UMI labelled, stranded libraries for whole transcriptome analysis using Illumina® next generation sequencing (NGS) platforms. With built-in UMIs and 384 UDIs for maximised sequencing output, and an all-in-one protocol, this technology allows for complete and uniform transcript coverage from start to end, and flexible input requirements (1ng to 1 μg).
Benefits of using the CORALL Total RNA-seq V2 library prep kits
- Fast and easy library preparation with ready-to-sequence library within 4.5 hours
- Suitable for challenging samples (including FFPE and plant RNA)
- Automation-friendly
- Minimised bias with RNA fragmentation-free protocol
- Superior end-to-end coverage, including for low-abundance transcripts
- >99% strandedness
- Cost saving multiplexing
The CORALL Total RNA-Seq V2 Library Prep kits ensure end-to-end coverage
Read coverage was analysed using the ERCC spike-in controls, which feature precise, known transcription start (TSS) and end (TES) sites. The absolute nucleotide positions relative to the TSS (red dotted line, A) and TES (blue dotted line, B) are shown. CORALL reads map more accurately to the exact ERCC TSS (A) than competitor libraries, which fail to cover the true start sites. Additionally, CORALL provides elevated coverage at TES (B).
Applications of CORALL Total RNA-Seq
- Isoform discovery & quantification
- Alternative splicing studies
- Transcript (re)annotation
- Circular RNA analysis
- De novo assembly
- Gene expression profiling
- SLAMseq metabolic RNA sequencing
System compatibility
- HiSeq® 2000/2500/3000/4000
- NextSeq® 500/550/2000
- MiSeq®
- iSeq®
- NovaSeq® 6000
Material available for download
CORALL Total RNA-Seq V2 flyer
CORALL Total RNA-Seq user guide
Illumina, HiSeq, NextSeq, MiSeq, iSeq and NovaSeq are registered trademarks of Illumina, Inc.
Products
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