CORALL FFPE Whole Transcriptome RNA-Seq ensures optimal coverage for FFPE samples of various quantities and qualities, making it ideal for analysing even low-quality FFPE and Biobank samples with a DV200<10%*. The kit facilitates the rapid and cost-effective creation of stranded, UMI-labelled, and unique dual indexed libraries with customisable insert sizes. Complete workflow bundles are available, including rRNA depletion for human, mouse, and rat ribosomal transcripts, offering a convenient and comprehensive solution.
Paired with RiboCop for enzyme-free rRNA depletion, CORALL FFPE enables in-depth, comprehensive analysis of the entire transcriptome, covering all RNA molecules, including non-polyadenylated and long non-coding RNAs. It also facilitates isoform detection, analysis of fusion transcripts, alternative splicing events, transcriptome assembly, and more.
Benefits of using CORALL FFPE
- Optimised for degraded FFPE RNA samples
- UMI-labelled, and unique dual indexed libraries within 1 hour
- Compatible with standard NGS platforms
- Detect low abundance transcripts
- Facilitates isoform detection, analysis of fusion transcripts and alternative splicing events
Figure 1. CORALL FFPE whole transcriptome RNA-seq workflow.
Applications of CORALL FFPE
- Cancer research: tumour profiling, retrospective studies
- Clinical research: biomarker discovery, personalised medicine
- Pathology: molecular diagnostics, disease mechanism studies
- Pharmaceutical research: drug development, toxicology studies
- Translational research: comparative studies
* A DV200 score is the percentage of RNA fragments within your sample that are above 200 nucleotides in length. A low score means your sample is highly degraded, the kind of sample many library preparation kits struggle to generate libraries from. However, the CORALL FFPE whole transcriptome RNA-seq kit is optimised for such samples and ensures the best sequencing results from FFPE samples.
Products
Note: product availability depends on country - see product detail page.
