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CytoSelect™ 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

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96 assays
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£624.00 Estimated delivery Wed 8 Oct
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Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect™ 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

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CBA-320 Assay Scheme
CBA-320 Assay Scheme
HSC Colony Formation. Human bone marrow
HSC Colony Formation. Human bone marrow derived CD34+ Hematopoietic progenitor cells were seeded at 3000 cells/well and cultured for 7-10 days in the absence or presence of growth factors/cytokines. Colonies were quantified according to the assay protocol. A: After 7 days without cytokines. B: After 7 days in the presence of cytokines. C: After 10 days in the presence of cytokines (hemoglobin visible).