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VAChT Antibody

Catalogue number:
600-401-MK7
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Immunohistochemistry, IF Microscopy, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human); Rat

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Anti-VAChT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal port

Alternative Names:

Rabbit Anti-Vesicular acetylcholine transporter Antibody, VAChT, rVAT, solute carrier family 18 member 3, Slc18a3, Vacht, Vat

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Product Description:

Slc18a3 (Solute Carrier Family 18 Member A3) is a Protein Coding gene. Vesicular acetylcholine transporters (VAChTs) are members of the solute carrier family 18 (SLC18) of ATP-dependent transporters that also includes vesicular monoamine transporters (VMAT) 1 and VMAT2. VAChT is found in the central and peripheral nervous systems. Transports acetylcholine into secretory vesicles for release into the extracellular space. Anti-VAChT Antibody is useful for researchers interested in Myasthenic syndromes, neurotransmitter release cycles, synaptic vesicle cycle, and neuroscience research. Anti-Vesicular Acetylcholine Transporter Antibody has been tested in ELISA, WB, IHC, and IF. Expect a band at ~56.5kDa in western blot using appropriate lysates. Positive control used: PND2-6 in WB, PND1 cells in IF; rat spinal cord in IHC.

Immunofluorescence of Rabbit Anti-VAChT
Immunofluorescence of Rabbit Anti-VAChT Antibody. Cell Line: Post-natal rat pup (PND1) heterogeneous brain cells. Primary Antibody: Rabbit Anti-VAChT Antibody at 15µg/mL overnight at 4°C. Counterstain Antibody: Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated (p/n 611-741-127) at 5µg/mL for 1hr at RT. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Nuclear Counter Stain: DAPI. Image: A) DAPI (blue). B) Anti-VAChT (green) and Secondary. C) Merge of A+B images. D) Secondary only. Localization: predicted membrane, Golgi apparatus. Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed Slc18a3 staining in mouse brain P7 Gensat project: http://www.gensat.org/imagenavigator.jsp?imageID=66611
Immunohistochemistry of Rabbit Anti-VACh
Immunohistochemistry of Rabbit Anti-VAChT Antibody. Tissue: Rat Spinal Cord. Primary Antibody: Rabbit Anti-VAChT Antibody at 1:100 for 30min at RT. Secondary Antibody: Anti-Rabbit Poly-HRP-IgG. Ready to use 8 min at RT. Antigen Retrieval: HIER using Citrate Buffer for 20 min. Counterstain: Hematoxylin.