VDAC/Porin Antibody

VDAC/Porin Antibody recognizes VDAC (also known as Voltage-dependent anion-selective channel protein 1) which is an outer membrane mitochondrial protein. The VDAC proteins are ~30-33 kDa (some isoforms are larger) . The VDAC proteins are thought to form aqueous channels, or pores, through which adenine nucleotides cross the outer mitochondrial membrane. VDACs have been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, adenine nucleotide translocator (ANT) , and cyclophilin D (CypD) , is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis. VDACs are highly expressed in heart, liver and skeletal muscle, where concentrations of mitochondria are at their highest. Diseases associated with VDAC1 include Cystic Fibrosis and Gerstmann-Straussler Disease. This antibody can be used as a loading control with whole cell lysates and total mitochondrial preparations. Anti-VDAC/Porin Antibody is useful for researchers interested in Apoptosis, cell survival and regulation, Mitochondrial Proteins, and Neuroscience Research. VDAC/Porin Antibody has been tested for use in ELISA, western blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30-33 kDa in size corresponding to VDAC/Porin by western blotting in the appropriate cell lysate or extract. Positive control used in WB: HeLa Whole Cell, Mouse Heart Lysate, or Rat Brain Tissue Lysate, and HeLa Cells in IF.