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HEY1 Antibody

Catalogue number:
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600-401-HB5S
Supplier:
Rockland Immunochemicals
Size:
25 µl
Product is available in:
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£146.00
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human); Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal porti

Alternative Names:

Rabbit Anti-HEY1 Antibody, BHLHB31, CHF2, HERP2, HESR1, HRT1, Hairy/Enhancer-Of-Split Related With YRPW Motif 1, Hes Related Family BHLH Transcription Factor With YRPW Motif 1, Hairy And Enhancer Of Split-Related Protein 1, Class B Basic Helix-Loop-Helix

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Product Description:

HEY1 belongs to the hairy and enhancer of split-related family (HESR) , a transcriptional repressor family. This nuclear protein is induced by the Notch pathway and is mainly associated with cardiovascular development. This family is known to repress GATA4, GATA6, MASH1, and NEUROD4 downstream effects. It’s role in liver cancer cell self-renewal is still being studied, as it’s suspected role in neurogenesis in mice. Hey1 is associated with diseases such as mesenchymal and periosteal chondrosarcoma. Anti-HEY1 antibody is useful for researchers interested in cancer research, neuroscience research, and Notch signalling. Anti-HEY1 Antibody has been tested in Western Blot, ELISA, and Immunohistochemistry. Expect a band at ~33 kDa in western blot using appropriate tissues or lysates. Positive control used is a recombinant human Hey1 at 1µg/mL for WB; human cerebellum tissue at 1:100 for IHC.

Immunohistochemistry of Rabbit Anti-HEY1
Immunohistochemistry of Rabbit Anti-HEY1 Antibody. Tissue: Human Cerebellum. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti-HEY1 at 1:200 for 30 minutes at RT. Secondary Antibody: Ready-to-Use Anti-Rabbit IgG HRP for 8 minutes at RT. Counterstain: Hematoxylin. Results: HEY1 showed diffuse cytoplasmic/membranous staining of low to moderate intensity of cells in the molecular layer, Pukinje cells layer, and granular cell layer in human cerebellum.
Western Blot of Rabbit Anti-HEY1 Antibod
Western Blot of Rabbit Anti-HEY1 Antibody. Lane 1: Opal PreStained Molecular Weight Marker (p/n Mb-210-0500). Lane 2: HEY1 variant 2 human recombinant protein (50ng) [+]. Lane 3: A-172 lysate (p/n W09-001-GL5) (35µg) [+]. Lane 4: Jurkat lysate (p/n W09-001-370) (35µg) [+]. Lane 5: Mouse brain lysate (p/n W10-000-T004) (35µg). Primary Antibody: Anti-HEY1 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG [H&L] Mx10 HRP (p/n 611-103-122) at 1:70,000 for 30 minutes at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~37kDa recombinant, ~33kDa endogenous. Observed: ~35-38kDa. Exposure: 19 sec.