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ProXtract B™ Extraction Buffer

Catalogue number:
MB-087-0100
Size:
100 ml
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Purity:

ProXtract B Extraction Buffer was aseptically filt

Formulation:

Liquid (sterile filtered)

Alternative Names:

ProXtract B™ Extraction Buffer, Protein Extraction Buffer, Bacterial Protein Extraction Reagent, Bacterial cell lysis buffer

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Product Description:

ProXtract B™ is ready-to-use as a working 1X solution and requires no further dilution. This buffer can be stored and used at room temperature. ProXtract B™ Extraction Buffer is gentle and has a high efficiency of protein recovery. It is compatible with chelating agents, reducing agents, protease inhibitors, cocktail inhibitors and chaotropic salts. There is no interference with downstream purification processes. Other applications may require detergents or other additional components. ProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.

SDS-PAGE of Construct A, B and C plasmid
SDS-PAGE of Construct A, B and C plasmids transformed in E.Coli BL21 strains. After overexpression, cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Supernatant of Construct A. Lane 2: Pellet from Construct A. Lane 3: Supernatant of Construct B. Lane 4: Pellet from Construct B. Lane 5: Supernatant of Construct C. Lane 6: Pellet from Construct C.
SDS-PAGE of recombinant protein D/ pGEX-
SDS-PAGE of recombinant protein D/ pGEX-2T plasmid transformed in Rosetta strain. Cells were plated on LB Cam-Amp, grown, and induced with IPTG. Cells were pelleted by centrifugation at 5000 rpm for 10 mins. Protein extraction was performed with ProXtract buffer for 20 mins at RT. Centrifugation is then done at 12000 rpm for 20 minutes at 4⁰C. Lane 1: Pellet from 1h. Lane 2: Supernatant of 1h. Lane 3: Pellet from 2h. Lane 4: Supernatant of 2h. Lane 5: Pellet from 3h. Lane 6: Supernatant of 3h.