2X SDS-PAGE Sample Buffer
Sample buffer for protein electrophoresis.
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. 2X SDS-PAGE Sample Loading Buffer is suitable for laboratory involved in protein biochemistry. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. The pH of this solution is 6.8. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.