SEPHAROSE™ Protein A

Sepharose™ Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative) . The bead size is approximately 45-165 µm when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.