SEPHAROSE™ Protein A/G

Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. Sepharose™ Protein A/G is a suspension of sepharose beads containing binding domains from both protein A (from Staphylococcus aureus) and protein G (from Streptococcus sp.) . When evenly dispersed approximately 2.0 ml of suspension is equal to 0.5 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative) . The bead size is approximately 45-165 µm when swollen. Each cc of drained beads will bind approximately 20-30 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Up to 100 reactions per 2ml. Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.