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rMouse SDF-1 alpha/CXCL12 Protein

Catalogue number:
010-001-W22-0100
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
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Purity:

Stromal Cell-Derived Factor-1 alpha (CXCL12) purit

Alternative Names:

12-O-tetradecanoylphorbol 13-acetate repressed protein 1 (TPAR1), C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor (PBSF), Thymic lymphoma cell-stimulating factor (TLSF)

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Product Description:

Stromal Cell Derived Factor-1 alpha (SDF-1α) , also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1α signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human and mouse SDF-1α share 99% sequence identity. Recombinant mouse SDF-1α is a non-glycosylated protein, containing 68 amino acids, with a molecular weight of 7.9 kDa. Stromal Cell-Derived Factor-1 alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stromal Cell-Derived Factor-1 alpha in immunological assays.

SDS-PAGE of Mouse Stromal Cell-Derived F
SDS-PAGE of Mouse Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein. Lane 1: 1 µg Mouse SDF-1 alpha in non-reducing conditions (-). Lane 2: Molecular weight marker. Lane 3: 1 µg Mouse SDF-1 alpha in reducing conditions (+). Lane 4: Molecular weight marker. Mouse SDF-1 alpha has a predicted MW of 7.9 kDa.
Bioactivity of Mouse Stromal Cell-Derive
Bioactivity of Mouse Stromal Cell-Derived Factor-1 alpha (CXCL12) Recombinant Protein. Samples of primary naïve human CD3+ T cells were allowed to migrate to Mouse SDF-1α/CXCL12 (0, 5, 100, and 500 ng/mL). After 4 hours, cells that migrated were counted using a fluorescent substrate and displayed on the bar graph above. Increased levels of migration over basal were seen in response to Mouse SDF-1α/CXCL12 starting at 5 ng/mL. This result is comparable to the expected range of 10 ng/mL.