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rMouse NGF beta Protein

Catalogue number:
010-001-W03-0100
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
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Purity:

Nerve Growth Factor beta purity was determined to

Alternative Names:

beta-NGF

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Product Description:

Nerve Growth Factor (NGF-β) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-β acts through its receptor β-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-β also is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant mouse NGF-β is a non-glycosylated, non-covalently linked homodimer, containing two 120 amino acid chains , with a molecular weight of 13.5 kDa each. Nerve Growth Factor beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Nerve Growth Factor beta in immunological assays.

SDS-PAGE of Mouse Nerve Growth Factor be
SDS-PAGE of Mouse Nerve Growth Factor beta Recombinant Protein. Lane 1: 1 µg Mouse NGF beta in non-reducing conditions (-). Lane 2: 1 µg Mouse NGF beta in reducing conditions (+). Lane 3: Molecular weight marker. Mouse NGF-beta is a homodimer with a predicted MW totaling 13.5 kDa.
Bioactivity of Mouse Nerve Growth Factor
Bioactivity of Mouse Nerve Growth Factor beta Recombinant Protein. Serial dilutions of Mouse β NGF, starting at 100 ng/mL, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of Mouse NGF is between 0.3-0.4 ng/mL. This value is comparable to the typical expected range of less than 1 ng/mL.