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  3. rMouse CCL2 Protein
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rMouse CCL2 Protein

Catalogue number:
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010-001-V74-0100
Supplier:
Rockland Immunochemicals
Size:
100 µg
Product is available in:
  • UK
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£767.00
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Purity:

Monocyte Chemotactic protein-1 (CCL2) purity was d

Alternative Names:

JE, MCAF

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Product Description:

Monocyte Chemotactic Protein 1 (MCP-1) , also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Recombinant mouse MCP-1 is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 13.8 kDa. Monocyte Chemotactic Protein-1 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Monocyte Chemotactic Protein-1 in immunological assays.

SDS-PAGE of Mouse Monocyte Chemotactic P
SDS-PAGE of Mouse Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein. Lane 1: Molecular weight marker. Lane 2: 1 µg Mouse MCP-1 in non-reducing conditions (-). Lane 3: 1 µg Mouse MCP-1 in reducing conditions (+). Mouse MCP-1 has a predicted MW of 13.8 kDa.
Bioactivity of Mouse Monocyte Chemotacti
Bioactivity of Mouse Monocyte Chemotactic Protein-1 (CCL2) Recombinant Protein. Human THP-1 cells were allowed to migrate to Mouse MCP-1 at (0, 0.1, 1, 10, 100 and 1000 ng/mL). After 45 minutes, cells that migrated were counted using a luminescent substrate and displayed on the bar graph above. Significant increases in migration over basal levels were seen in response to Mouse MCP-1 starting at 1 ng/mL. This value is comparable to expected ranges of a chemotactic response of primary human monocytes.