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  3. Green Fluorescent Protein (GFP) Control Protein
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Green Fluorescent Protein (GFP) Control Protein

Catalogue number:
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000-001-215
Supplier:
Rockland Immunochemicals
Size:
100 µg
Product is available in:
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£505.00
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Purity:

GFP control protein was expressed in E. coli and p

Alternative Names:

control protein, rGFP protein, GFP fusion protein, GFP control

Product Description:

Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in Anti-GFP antibody assays. Rockland offers multiple purified hosts and conjugated Anti-GFP that can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal Anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal Anti-GFP (p/n 600-301-215) , using either form of the antibody as the capture or detection antibody. GFP Control is suitable as a control for polyclonal or monoclonal Anti-GFP in immunological assays. Researchers should determine optimal titers for applications. The molecular weight of GFP is 28 kDa. Green Fluorescent Protein has been tested in SDS-page, western blot, and immunoprecipitation.

Western Blot of anti-GFP monoclonal anti
Western Blot of anti-GFP monoclonal antibody. Lane 1: 64pg of recombinant GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364). Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. Block: TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C. Predicted/Observed size: 27 kDa for GFP. Other band(s): none.