Maltose Binding Protein (MBP) Control Protein

Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. Maltose-Binding Protein is suitable for use as a control in many assays including affinity purification, western blot and other biological assays. Optimal concentration should be determined by the researcher. Expect MW of MBP~51kDa consisting of multiple cloning sites from vector (MCS) and the MBP; molecular weight of MBP at ~40kDa. Maltose-Binding Protein has been tested in SDS-page and western blot.