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Rabbit IgG F(ab')2

Catalogue number:
Size:
2 mg
Product is available in:
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Purity:

Rabbit IgG F(ab')2 was prepared from normal serum

Alternative Names:

Rabbit Immunoglobulin IgG F(ab')2 fragment, IgG Fab2

Product Description:

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them) , activation of the compliment cascade, and opsonization for phagocytosis. This product possesses the F (ab') 2 fragment, recognized by the two F (ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region. Rabbit IgG F (ab') 2 Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

SDS-PAGE of Rabbit IgG F(ab')2 Fragment
SDS-PAGE of Rabbit IgG F(ab')2 Fragment (p/n 011-0104). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule (p/n 011-0102). Lane 2: Reduced Rabbit IgG F(ab')2 Fragment (p/n 011-0104). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab')2 and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.