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Mouse IgG1 (Gamma 1 chain) ATTO 647N Conjugated Antibody

Catalogue number:
610-156-040
Size:
500 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

FLISA, Flow Cytometry, IF Microscopy, Western Blot

Antibody Host:

Goat

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Mouse IgG1 heavy chain

Alternative Names:

Goat anti-mouse IgG1 antibody ATTO647N conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 647N conjugated antibody

Product Description:

Anti-Mouse IgG1 ATTO 647N Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain) . Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain constitutes 66% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG1 ATTO 647N Antibody has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

ATTO ® dyes can be used for multicolor i
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH