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Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP

Catalogue number:
18-8817-33
Size:
200 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£301.00 Estimated delivery Thu 4 Apr
Shipping is calculated in checkout
Applications:

IF Microscopy, Immunoprecipitation, Western Blot

Antibody Host:

Rat

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

Alternative Names:

Anti-Mouse IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting

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Product Description:

Mouse IgG TrueBlot® ULTRA is a unique Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western blot data with Mouse IgG TrueBlot® ULTRA, simply substitute the conventional Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. Mouse IgG TrueBlot® ULTRA preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Mouse TrueBlot® ULTRA has been tested in ELISA, IP/immunoblot, and immunoblotting (WB) . Mouse IgG TrueBlot® ULTRA is provided as 1000X solution. To achieve best results when detecting mouse IgG1 subtypes, we recommend performing a dot blot or titration to determine the optimal dilution factor for your desired application. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs) . If used conservatively at 2.5mls/blot will yield enough reagent for 80 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries, but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.

Storage Temperature:

Store at -20°C. This product is guaranteed for 1 year upon receipt, when handled and stored as instr

Mouse TrueBlot® IP / Western Blot:
Caspa
Mouse TrueBlot® IP / Western Blot: Caspase 7 was immunoprecipitated from 0.5 ml of 1x10e7 Jurkat cells/ml with 5 ug mouse anti-human Caspase 7. Precipitate from 1x10e6 cells was subjected to electrophoresis, transferred to an PVDF membrane, and Western blotted with anti-Caspase 7 using Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP (Lane 1) or conventional HRP-conjugated anti-mouse antibody (Lane 2) - note the detection of the heavy and light chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1. When Lane 1 is re-immunoblotted using conventional HRP-conjugated anti-mouse polyclonal antibody (Lane 3), the heavy and light chains are now detected, confirming that although the immunoprecipitating heavy and light chains are present, Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP detects only native antibody and not denatured heavy and light chains.
Western Blot of Mouse TrueBlot® ULTRA: A
Western Blot of Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP. Lane 1: Mouse IgG, non-denatured. Lane 2: 5 µL Opal Pre-stained Ladder (p/n MB210-0500). Lane 3: Mouse IgG, denatured. Load: 50 ng. Primary antibody: none. Secondary antibody: Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 160 kDa non-denatured for Mouse IgG.
Details Cat number & supplier Size Price
Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP 18-8817-30 · Rockland Immunochemicals
18-8817-30
Rockland Immunochemicals
20 µl £77.00
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Mouse TrueBlot®: Anti-Mouse Ig Biotin 13-8817-80 · Rockland Immunochemicals
13-8817-80
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Goat TrueBlot®: Anti-Goat IgG HRP 18-8814-31 · Rockland Immunochemicals
18-8814-31
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50 µl £150.00
50 µl
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Sheep TrueBlot®: Anti-Sheep IgG HRP 18-8815-31 · Rockland Immunochemicals
18-8815-31
Rockland Immunochemicals
50 µl £150.00
50 µl
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Rabbit TrueBlot®: Anti-Rabbit IgG HRP 18-8816-31 · Rockland Immunochemicals
18-8816-31
Rockland Immunochemicals
50 µl £150.00
50 µl
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Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP 18-8817-31 · Rockland Immunochemicals
18-8817-31
Rockland Immunochemicals
50 µl £150.00
50 µl
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Mouse TrueBlot®: Anti-Mouse Ig Biotin 13-8817-82 · Rockland Immunochemicals
13-8817-82
Rockland Immunochemicals
100 µg £270.00
100 µg
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Goat TrueBlot®: Anti-Goat IgG HRP 18-8814-33 · Rockland Immunochemicals
18-8814-33
Rockland Immunochemicals
200 µl £301.00
200 µl
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Sheep TrueBlot®: Anti-Sheep IgG HRP 18-8815-33 · Rockland Immunochemicals
18-8815-33
Rockland Immunochemicals
200 µl £301.00
200 µl
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Rabbit TrueBlot®: Anti-Rabbit IgG HRP 18-8816-33 · Rockland Immunochemicals
18-8816-33
Rockland Immunochemicals
200 µl £301.00
200 µl
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