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Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed

Catalogue number:
609-106-017
Size:
1 mg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Goat

Species Reactivity:

H. sapiens (Human)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Human IgG F(c) fragment

Alternative Names:

Goat Anti Human IgG Fc Biotin Conjugation Antibody, Goat Anti-Human IgG F(c) Antibody Biotin Conjugated, Goat Anti Human IgG Fc Fragment Antibody Biotin Conjugate

Product Description:

Anti-Human IgG F (c) Biotin Conjugated generated in goat detects Human F (c) . A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F (c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Anti-Human IgG F (c) Biotin Conjugated has been tested by ELISA and is designed for Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods.

ELISA results of purified Goat anti-Huma
ELISA results of purified Goat anti-Human IgG F(c) Antibody Biotin conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated (p/n S000-03) and TMB substrate p/n TMBE-1000.