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Mouse IgG (H&L) Antibody Pre-adsorbed

Catalogue number:
Size:
2 mg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit

Alternative Names:

rabbit anti-Mouse IgG Antibody, Rabbit-a-Mouse IgG (H&L), Mouse IgG Antibody in rabbit

Product Description:

Anti-Mouse IgG antibody generated in rabbit detects specifically mouse IgG (H&L) . Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them) , activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Mouse secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Mouse IgG (H&L) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot) , ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA results of purified Rabbit anti-Mo
ELISA results of purified Rabbit anti-Mouse IgG Antibody (min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 009-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin, Goat anti-Rabbit igG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB ELISA Substrate (p/n TMBE-1000).