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Sheep TrueBlot®: Anti-Sheep IgG HRP

Catalogue number:
18-8815-31
Size:
50 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£150.00 Estimated delivery Thu 4 Apr
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Applications:

Western Blot

Antibody Host:

Mouse

Species Reactivity:

Sheep

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

Alternative Names:

Anti-Sheep IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting

Product Description:

Sheep IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Sheep IgG monoclonal secondary antibody. Sheep IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Sheep IgG TrueBlot®, simply substitute the conventional HRP Anti-Sheep IgG blotting reagent with Sheep IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Sheep IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of Sheep IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Sheep IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Sheep IgG HRP TrueBlot has been tested in western blot and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Sheep IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs) . If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the Mouse, Goat and Sheep TrueBlot secondaries, but not with the Rabbit TrueBlot secondary. Use of protein A or G beads with the Rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.

Storage Temperature:

Store at -20°C. This product is guaranteed for 1 year upon receipt, when handled and stored as instr

Sheep TrueBlot® IP / Western Blot:  
Jur
Sheep TrueBlot® IP / Western Blot: Jurkat cell lysate (500 µg) was incubated with 2 µg of sheep anti-SLP76 and immunoprecipitated using Protein G. 20 µg Jurkat lysate (lane 1), immunoprecipitate (lane 2) and 1.0 µg and 0.1.0 µg reduced anti-SLP76 (lanes 3 and 4) were subjected to electrophoresis, transferred to a PVDF membrane, and immunoblotted with anti-SLP76 using conventional HRP-conjugated anti-sheep polyclonal antibody (left panel) and Sheep TrueBlot®: Anti-Sheep IgG HRP (right panel).
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
A90-216P
Bethyl Laboratories, Inc.
0.5 mg £124.00
0.5 mg
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