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Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Catalogue number:
610-1319-0500
Size:
500 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Goat

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Mouse IgG whole molecule

Alternative Names:

Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horserad

Product Description:

Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them) , activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting, as well as other peroxidase antibody based assays.

ELISA results of purified Goat anti-Mous
ELISA results of purified Goat anti-Mouse IgG Antibody Peroxidase Conjugated (Min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 009-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TME ELISA Peroxidase Substrate (p/n TMBE-1000).