www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Human Serum Albumin Antibody

Catalogue number:
600-401-033
Size:
1 mg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£1547.00 Shipping is calculated in checkout
Applications:

ELISA, Immunohistochemistry, IF Microscopy, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Human Serum Albumin

Alternative Names:

rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody

image 0
image 1
Product Description:

Anti-Human Serum Albumin Antibody detects serum albumin which is the most abundant protein in human blood plasma. Serum albumin is produced in the liver and constitutes about half of the blood serum protein. It is soluble and monomeric, and is shown to transport hormones, fatty acids, and other compounds, buffers pH, and maintains osmotic pressure, among other functions. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. The reference range for albumin concentrations in blood is 3.4 to 5.4 g/dL. Serum Albimin has a molecular mass of 67 kDa. This antibody is suitable for researchers in cardiovascular research, Serum Protein Component research, as well as general research. Anti-Human Serum Albumin Antibody has been tested by ELISA, SDS-PAGE, dot blot, and western blot. This product is suitable for the detection of human serum albumin in blood and tissue samples. Antibody is suitable for western blotting to detect a single band of 67 kDa, the expected apparent molecular weight, and for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot of human serum albumin.   L
Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. Dylight 649 conjugated Stretavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.
Dot Blot of Rabbit IgG protein. Antigen:
Dot Blot of Rabbit IgG protein. Antigen: control Rabbit IgG protein. Load: 250ng starting total load in a serial 1:1 dilution. Primary antibody: Rabbit IgG protein. Secondary antibody: Rabbit IgG Antibody Peroxidase Conjugated and Rabbit IgG Antibody DyLight™ 649 Conjugated secondary antibodies at 1:20,000 for 45 min at RT. Block: MB-070 buffer overnight at 4°C. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager.