F4/80 Fluorescein Antibody
Flow Cytometry
Rat
Mus musculus (Mouse)
IgG2a
Monoclonal
Anti-F4/80 Antibody (Monoclonal) was produced by repeated immunizations with F4/80 antigen.
EMR1, Ly71, EGF-TM7, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Gpf480, Adgre1
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. BM8.1 is widely used together with antibodies to CD115 (c-fms) , CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry. Anti-F4/80 is tested for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated) . Researchers should determine optimal titers for applications that are not stated.
