Anti-DYKDDDDK (FLAG® tag) Affinity Gel

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means. Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG® and HA. Anti-DYKDDDDK Affinity Gel has been tested by SDS-Page, IP, and western blot and is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins. Anti-DYKDDDDK Affinity Gel antibody recognizes the FLAG® epitope tag fused to either the amino- or carboxy- terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol) . Anti-FLAG® is a registered trademark of Sigma-Aldrich. Refer to the Protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.