c-erbB2 Antibody

The ERBB2 gene encodes a member of the tyrosine protein kinase superfamily that is an essential component of a neuregulin-receptor complex called c-erbB2 (also known as Receptor tyrosine-protein kinase erbB-2, p185erbB2, C-erbB-2, NEU proto-oncogene, tyrosine kinase-type cell surface receptor HER2, MLN 19 and CD340) , although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. C-erbB2 is not activated by EGF, TGF-alpha and amphiregulin. Defects in ERBB2 are associated with gastric cancer, familial glioma of brain (glioblastoma multiforme) , ovarian cancer and lung cancer (adenocarcinoma of lung) . c-erbB2 antibody is ideal for Cancer and Signal Transduction research. Anti-erbB2 Antibody monoclonal antibody contains TBS/1% BSA. Anti-erbB2 Antibody is tested with IHC and is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 185 kDa in size corresponding to c-erbB2 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min. C-erbB2 is found in human breast carcinoma tissue at the cell membrane.