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MAD2L2 Antibody

Catalogue number:
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600-401-470
Supplier:
Rockland Immunochemicals
Size:
100 µg
Product is available in:
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£580.00
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N

Alternative Names:

rabbit anti-MAD2L2 antibody, MAD2B, REV7, hREV7, REV homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B

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Product Description:

MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein. MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. For western blotting HeLa whole cell lysates and nuclear extracts are suggested. Reactivity in other immunoassays is unknown. Antibody activity can be blocked using a control peptide (000-001-470) .

Rockland's Affinity Purified anti-MAD2L2
Rockland's Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
Affinity Purified Rabbit anti-MAD2L2 was
Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.