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RFX5 Antibody

Catalogue number:
200-401-194
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Chip, Gel Shift, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Alternative Names:

rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

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Product Description:

Anti-RFX5 is specific for the RFX5 protein. RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. RFX5 has been shown to interact with CIITA. Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.

Western Blot of Rabbit Anti-RFX5 antibod
Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.
Western Blot of Rabbit Anti-RFX5 antibod
Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Hela Nuclear Extract. Load: 25 µg per lane. Primary antibody: RFX5 antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 65.3 kDa, 60 kDa for RFX5.