SIX3 Antibody

Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain. Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product. Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke’s pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary. This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.