p16 Antibody

The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12) , two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF) , with the first exon located 20kb upstream of the remainder of the gene (isoform 4 a.k.a. p14ARF, p19ARF, ARF) . In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. This product was tested by immunoblot and found to be reactive against p16 from cell lysates and E.coli produced fusion proteins at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (code #611-1302) . Anti-p16 is suitable for the detection by immunoblot of human p16. This antisera is also suitable for immunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5. This product was tested in IHC.