www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

ERK-MAPK phospho T202/phospho Y204 Antibody

Catalogue number:
600-401-C76
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£717.00 Shipping is calculated in checkout
Applications:

Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human); Mus musculus (Mouse); Rat

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Anti-ERK/MAPK Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surroundin

Alternative Names:

Mitogen-activated protein kinase 1, MAP kinase 1, MAPK1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42

Product Description:

Anti-ERK/MAPK pT202/pY204 Antibody detects ERK/MAPK which is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204. Anti-ERK/MAPK pT202/pY204 Antibody is ideal for investigators involved in Cell Signaling and Cancer Research. Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42-44 kDa in size corresponding to ERK/MAPK phosphorylated at Thr202 and Tyr204.

Western Blot of Rabbit anti-ERK-MAPK Thr
Western Blot of Rabbit anti-ERK-MAPK Thr202-Tyr204 Antibody. Lane 1: human T47D cell lysates. Lane 2: human T47D cell lysates incubated in λ-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: ERK-MAPK Thr202-Tyr204 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~42-44kDa for ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Other band(s): none.