www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

ChAT (Choline Acetyltransferase) Antibody

Catalogue number:
600-101-D16
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£696.00 Shipping is calculated in checkout
Applications:

Immunohistochemistry, Western Blot

Antibody Host:

Goat

Species Reactivity:

Avian; Cavia porcellus (Guinea Pig); Chicken; H. sapiens (Human); Mus musculus (Mouse); Non-Human primates

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Alternative Names:

acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Product Description:

ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research. Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Western Blot of Goat Anti-Choline Acetyl
Western Blot of Goat Anti-Choline Acetyltransferase Antibody. Lane 1: rat brain lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: ChAT antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ goat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~70kDa/~70kDa for ChAT. Other band(s): none.