Myelin Basic Protein (MBP) Antibody

Myelin Basic Protein antibody detects MBP. MBP is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells. In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22kDa and another at about 18kDa. Anti-MBP antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research. Anti-Myelin Basic Protein antibody is tested for use in Western Blotting and IF. Expect a bands of approximately 18 kDa and 22 kDa in size corresponding to MBP proteins in Western blots of rat brain homogenate. Specific conditions for reactivity should be optimized by the end user.