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Streptavidin Antibody Fluorescein Conjugated

Catalogue number:
200-402-095
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

FLISA, Flow Cytometry, IF Microscopy

Antibody Host:

Rabbit

Species Reactivity:

Streptomyces avidinii

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Streptavidin (Streptomyces avidinii)

Alternative Names:

rabbit anti-Streptavidin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin FITC Antibody

Product Description:

Streptavidin antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7) . With a dissociation constant (Kd) on the order of ≈10−14 mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride) , detergents (e.g. SDS, Triton) , proteolytic enzymes, and extremes of temperature and pH. Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research. Anti-Streptavidin Antibody Fluorescein Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Rockland Rabbit anti Streptavidin (200-4
Rockland Rabbit anti Streptavidin (200-4195 lot 23495) and Biotin conjugated Rabbit anti-trypsin inhibitor antibody (200-4679 lot 6594) were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (611-743-127 lot 20831 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc System