CD45 Fluorescein Antibody

The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA) . CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220) , CD45RA, CD45RB, CD45RO and others. The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets. Anti-CD45 is tested for Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot using mouse spleen cells, or an appropriate cell type (where indicated) . Researchers should determine optimal titers for applications that are not stated.