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Pax7 Antibody

Catalogue number:
600-401-GR7S
Size:
25 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human); Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an i

Alternative Names:

rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7

Product Description:

The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA) . Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research. Anti-Pax7 antibody has been tested in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract.

Western Blot of Rabbit anti-Pax7 antibod
Western Blot of Rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: Mouse muscle protein lysate. Lane 3: C2C12. Lane 4: Ms spleen. Lane 5: Ms brain. Lane 6: HEK293T. Lane 7: HeLa WCL. Lane 8: MCF7. Lane 9: Jurkat. Lane 10: A431. Lane 11: A549. Lane 12: LNCap. Lane 13: MOLT-4. Lane 14: Ramos. Lane 15: Raji. Lane 16: Opal Pre-stained ladder (p/n MB-210-0500). Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for Pax7.