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  3. OspC Antibody
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OspC Antibody

Catalogue number:
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200-401-C11S
Supplier:
Rockland Immunochemicals
Size:
25 µl
Product is available in:
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£146.00
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Applications:

ELISA, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

Borrelia burgdorferi

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein.

Alternative Names:

rabbit anti-OspC Antibody, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, BB_B19

Product Description:

Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane. This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Western blot showing detection of 0.1 µg
Western blot showing detection of 0.1 µg of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein (arrow; expected MW: 63.1 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc™ MP 4000 imaging system (Bio-Rad).