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ALG6 Antibody

Catalogue number:
100-401-GJ0S
Size:
25 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

H. sapiens (Human)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of human ALG6 p

Alternative Names:

rabbit anti-ALG6 Antibody, rabbit anti-ALG-6 Antibody, Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase, Asparagine-linked glycosylation protein 6 homolog, Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase, Dolichyl-P-Glc:Man9

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Product Description:

Anti-ALG6 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA) . Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase is an enzyme that is encoded by the ALG6 gene in humans. This gene encodes ALG6/ALG8 member of glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type. Anti-ALG6 is ideal for researchers interested in Infectious disease, especially HIV and hepatitis virus infections. Anti-ALG6 antibody is applicable to methods for diagnosis and, in particular, to methods for diagnosing infections using biomarkers targeting exosomes secreted in bodily fluids. Anti-ALG6 antibody has been tested in ELISA, western blot, and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58kDa in size corresponding to ALG6 protein by Western blotting in the appropriate cell lysate or extract.

Immunohistochemistry with anti-ALG6 anti
Immunohistochemistry with anti-ALG6 antibody showing ALG6 staining in the squamous epithelium of the uterine cervix as well as in inflammatory elements diffused in the stroma of human cervical cancer tissue at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human ALG6 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).
Western Blot of Rabbit anti-ALG6 antibod
Western Blot of Rabbit anti-ALG6 antibody. Lane 1: HeLa Whole Cell lysate. Lane 2: MOLT4 Whole Cell lysate. Lane 3: K-562 Whole Cell lysate. Load: 10 µg per lane. Primary antibody: ALG6 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: TBS-Casein overnight at 4°C. Predicted/Observed size: 58 kDa ALG-6.