GAPDH Antibody

GAPDH loading control antibody is ideal for Western Blotting, ELISA, IHC and IF Microscopy. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control antibody in immunoblots. A loading control antibody is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) , an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease. Anti-GAPDH antibody is ideal for investigators involved in apoptosis, cancer, DNA damage and repair and neuroscience. Anti-GAPDH Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~36 kDa in size corresponding to GAPDH by western blotting in the appropriate cell lysate or extract. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, making it ideal for use as a loading control marker. This Anti-GAPDH Antibody product is a new_product replacing p/n 600-401-A33S.