www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F]

Catalogue number:
A700-028-T
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Fri 26 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, IHC-IF, Flow Cyt

Antibody Host:

Rabbit

Target:

LAG3

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

residues 23-450 (ECD)

Alternative Names:

lymphocyte-activation gene 3; Protein FDC; lymphocyte activation gene 3 protein; LAG-3; CD223; CD antigen CD223

image 0
image 1
image 2
Product Description:

Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR028F] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human LAG3 by immunohistoch
Detection of human LAG3 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] (A700-028-T lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human LAG3 by immunocytoche
Detection of human LAG3 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] (A700-028-T lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human LAG3 by western blot.
Detection of human LAG3 by western blot. Samples: Whole cell lysate (5 µg) from HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] (A700-028 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human LAG3 by western blot
Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] (A700-028-T lot 1) used for IP at 6 µl per reaction. LAG3 was also immunoprecipitated by rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] A700-027. For blotting immunoprecipitated LAG3, A700-028-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Details Cat number & supplier Size Price
SurePAGE™, Bis-Tris, 10x8, 4-12%, 12 wells M00653 · GenScript Biotech
M00653
GenScript Biotech
10 pk/box £56.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 4-20%, 12 wells M00656 · GenScript Biotech
M00656
GenScript Biotech
10 pk/box £56.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 10%, 12 wells M00665 · GenScript Biotech
M00665
GenScript Biotech
10 pk/box £56.00
10 pk/box
view