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Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR027F]

Catalogue number:
A700-027-T
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, IHC-IF, mIF

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 500 and 525 (C-term)

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Product Description:

Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR027F] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human LAG3 by immunohistoch
Detection of human LAG3 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (A700-027-T-1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.
Detection of human LAG3 by immunocytoche
Detection of human LAG3 by immunocytochemistry. Sample: FFPE section of HDLM-2. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (A700-027-T-1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substate: DAB.
Detection of human LAG3 by western blot.
Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 µg) from HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (A700-027-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human LAG3 by western blot
Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody [BLR027F] (A700-027-T lot 1) used for IP at 12 µl per reaction. LAG3 was also immunoprecipitated by rabbit anti-LAG3 recombinant monoclonal antibody [BLR028F] A700-028. For blotting immunoprecipitated LAG3, A700-027-T was used at 1:500. Chemiluminescence with an exposure time of 10 seconds.
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