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  3. A700-026-T
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Rabbit anti-B7-H3/CD276 Recombinant Monoclonal Antibody [BLR026F]

Catalogue number:
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A700-026-T
Supplier:
Bethyl Laboratories, Inc.
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 10 May
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Applications:

WB, IP, IHC, ICC, Flow Cyt

Antibody Host:

Rabbit

Target:

B7-H3/CD276

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

residues 29-465 (ECD)

Alternative Names:

B7RP-2; CD276 antigen; costimulatory molecule; CD antigen CD276; B7H3; 4Ig-B7-H3; B7 homolog 3; B7-H3

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Product Description:

Rabbit anti-B7-H3/CD276 Recombinant Monoclonal Antibody [BLR026F] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human B7-H3/CD276 by immuno
Detection of human B7-H3/CD276 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody [BLR026F] (A700-026 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
Detection of human B7-H3/CD276 by immuno
Detection of human B7-H3/CD276 by immunocytochemistry. Sample: FFPE section of A-172 cells. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody [BLR026F] (A700-026 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).
Detection of human B7-H3/CD276 by wester
Detection of human B7-H3/CD276 by western blot. Samples: Whole cell lysate (15 µg) from MCF-7, A-172, U-251MG, OVCAR-3, HeLa, 293T, HCT 116, RPMI-8226, MJ, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody [BLR026F] (A700-026-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (A300-639A).
Detection of human B7-H3/CD276 by wester
Detection of human B7-H3/CD276 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H3/CD276 recombinant monoclonal antibody [BLR026F] (A700-026-T lot 1) used for IP at 3 µl per reaction. B7-H3/CD276 was also immunoprecipitated by rabbit anti-B7-H3/CD276 recombinant monoclonal antibody [BLR025F] (A700-025). For blotting immunoprecipitated B7-H3/CD276, A700-026-T was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
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