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  3. A700-024-T
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Rabbit anti-hSET1 Recombinant Monoclonal Antibody [BLR024E]

Catalogue number:
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A700-024-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 24 May
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Applications:

WB, IP, IHC, ICC, IHC-IF

Antibody Host:

Rabbit

Target:

hSET1

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 1200 and 1250

Alternative Names:

Set1A; histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1; set1/Ash2 histone methyltransferase complex subunit SET1

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Product Description:

Rabbit anti-hSET1 Recombinant Monoclonal Antibody [BLR024E] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human and mouse hSET1 by we
Detection of human and mouse hSET1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] (A700-024-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes.
Detection of human hSET1 by western blot
Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] (A700-024-T lot 1) used for IP at 20 µl per reaction. hSET1 was also immunoprecipitated by rabbit anti-hSET1 antibody (A300-288A). For blotting immunoprecipitated hSET1, A700-024-T was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
Detection of human hSET1 by immunohistoc
Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma.  Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] (A700-024-T lot 1) used at 1:25. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-201D4).
Detection of human hSET1 by immunohistoc
Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [BLR024E] (A700-024-T lot 1) used at 1:25. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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